parallax background

stem cell terapy

جوان سازی پوست
Lifescell Group

International Bio-institute of Lifescell Co. , Established in 2005 , is the leader of biotechnology based on Tissue engineering and stem cell therapy

Lifescell group , serve an unmet need for better advanced Therapeutic , Cosmetic and , Bio-Supplement alternatives and products based on new born biotechnology science meets the highest Quality and clinical standards, provide innovative solutions according to FDA medical device guidelines and protocols of American Association of Tissue Banks (AATB)

LifesCell products have been produced maintaining all the beneficial features of placental allografts as amniotic & chorion membrane , Umbilical cord , and Placental cotyledon which contains high levels of growth factors (GFs) , cytokines , Mesenchymal stemcells (MCSCs) , Hyaluronic acid (crosslinked complex carbohydrate), Bio-active nutrients , Extra Cellular Matrix (ECM) composed of proteoglycan, glycosaminoglycan, glycoprotein, collagen (I, III, IV, VI, VIII), fibronectin, laminin, elastin, and much more regenerative bio elements for soft and hard tissue reconstruction & regenerating

Lifescell quality assurance is based on international standards like as ISO 13485/ 2016 , MOH /65273027 ,MOI/ 60556 , ISO 9001 , ISO 14001 , OHSAS 18001

Lifescell new born biotechnology

Life Cell production process is based on new born tissue engineering biotechnologic bio-implants production articles and done in Clean Rooms by Classification 100 , 1000 (According to FED STD 209E) . According to the AATB standard, After selecting the best quality donors , they are screened for chronic, infectious, neurological, autoimmune, malignant, and other environmental factors and the following tests are repeated for the patient after the Window Period (NAT tests) . Screening tests:

HIV-1 & HIV-2 Antibody -

HIV Type 1 (Nucleic Acid Test) -

HTLV-1 & HTLV-2 Antibody -

Hepatitis B Core Antibody -

Hepatitis B Surface Antigen -

Hepatitic C Antibody -

Hepatitic C Virus (Nucleic Acid Test) -

CMV Total Antibody -

Syphilis (Serological Test) -

dna_seq1 (Copy)
 
Lifescell products

Lifescell Bio-products , are categorized to three fields i.e

Therapeutic products *

Cosmetic products *

Bio-Supplements products *

What is Stem cell

stem cell

: Amniotic stem cells

Amniotic stem cells are the mixture of stem cells that can be obtained from the amniotic fluid as well as the amniotic membrane. They can develop into various tissue types including skin, cartilage, cardiac tissue, nerves, muscle, and bone. The cells also have potential medical applications, especially in organ regeneration

The stem cells are usually extracted from the amniotic sac by amniocentesis which occurs without harming the embryos. The use of amniotic fluid stem cells is therefore generally considered to lack the ethical problems associated with the use of cells from embryos

: Properties

The majority of stem cells present in the amniotic fluid share many characteristics, which suggests they may have a common origin

In 2007, it was confirmed that the amniotic fluid contains a heterogeneous mixture of multipotent cells after it was demonstrated that they were able to differentiate into cells from all three germ layers but they could not form teratomas following implantation into immunodeficient mice. This characteristic differentiates them from embryonic stem cells but indicates similarities with adult stem cells. However, foetal stem cells attained from the amniotic fluid are more stable and more plastic than their adult counterparts making it easier for them to be reprogrammed to a pluripotent state

A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. One of the more common isolation methods involves the removal of amniotic fluid by amniocentesis. The cells are then extracted from the fluid based on the presence of c-Kit. Several variations of this method exist. There is some debate whether c-Kit is a suitable marker to distinguish amniotic stem cells from other cell types because cells lacking c-Kit also display differentiation potential. Culture conditions may also be adjusted to promote the growth of a particular cell type

استم سل
محصولات استم سل انقلابی در علم پزشکی

: Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their isolation. They usually involve the removal of amniotic fluid by amniocentesis and their distinction from other cells may be based on their morphology or other characteristics

Human leukocyte antigen testing has been utilised to confirm that the MSCs stem from the fetus and not from the mother. Originally it was proposed that the MSCs were discarded from the embryo at the end of their life cycle but since the cells remained viable in the amniotic fluid and were able to proliferate in culture this hypothesis was overturned. However, it remains unclear whether the cells originate from the fetus itself, the placenta or possibly the inner cell mass of the blastocyst

Comparison of amniotic fluid-derived MSCs to bone-marrow-derived ones showed that the former has a higher expansion potential in culture. However, the cultured amniotic fluid-derived MSCs have a similar phenotype to both adult bone-marrow-derived MSCs and MSCs originating from second trimester fetal tissue. In animals, the MSCs seem to have a unique immunological profile which was observed after their isolation and in vitro culturing

: Embryonic-like stem cells

As opposed to mesenchymal stem cells, embryonic-like stem cells are not abundant in the amniotic fluid, making up less than 1% of amniocentesis samples. Embryonic-like stem cells were originally identified using markers common to embryonic stem cells such as nuclear Oct4, CD34, vimentin, alkaline phosphatase, stem cell factor and c-Kit. However, these markers were not necessarily concomitantly expressed. In addition, all of these markers can occur on their own or in some combination in other types of cells
The pluripotency of these embryonic-like stem cells remains to be fully established. Although those cells which expressed the markers were able to differentiate into muscle, adipogenic, osteogenic, nephrogenic, neural and endothelial cells, this did not necessarily occur from a homogenous population of undifferentiated cells. Evidence in favour of their embryonic stem cell nature is the cells’ ability to produce clones
Clinical applications

The use of amniotic stem cells instead of embryonic stem cells may be advantageous in some cases for various reasons including that the former do not form teratomas. Their enhanced stability and plasticity compared to adult stem cells may also be an advantage. Stem cells from both the amniotic fluid and membrane are utilised for therapeutic approaches